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. 2016 Dec 15;12(12):e1006232. doi: 10.1371/journal.pgen.1006232

Fig 6. The deletion of Hotair does not alter Hoxd genes expression in embryo.

Fig 6

(A) RNA-seq expression profiles of Hoxd genes in both the GT and T3 tissues of wild type (green) and Del(Hotair)-/- (orange) E12.5 embryos. The Y-axis represents the per-base read coverage, normalized by dividing by the total number of million mapped reads in the corresponding samples. The two biological replicates were pooled for this representation and only uniquely mapping reads were used. (B) WISH of Hoxd10 and Hoxd11 on E12.5 wild type (left) and Del(Hotair)-/- (right) embryos. The dashed lines indicate the rostral limits of the expression domains in the trunk, neural tube (black) and paraxial mesoderm (white). Adult vertebrae derive from the latter tissue. (C) Double WISH for the MyoD RNAs (for somite visualization) and either the Hoxd10 (upper panel) or Hoxd11 (lower panel) on E12.5 wild type (left) and Del(Hotair)-/- (right) embryos. There was no detectable difference in the anterior limit of expression for any Hoxd gene analyzed.