Fig 2. NF-YB2^E65R loses interaction with NF-YA subunits.

A) Alignment of the core domain of human and Arabidopsis NF-YB subunits. * marks the position of the conserved glutamic acid required for interaction with NF-YA in humans [27]. B) NF-YB2 and NF-YB2^E65R interact with NF-YC3, NF-YC4, and NF-YC9 in Y2H assays. C) NF-YB2, but not NF-YB2^E65R, interacts with NF-YA2 when NF-YC9 is expressed using a bridge vector in yeast three-hybrid assays. DBD: DNA binding domain, AD: activation domain, EV: empty vector control.