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. 2016 Dec 15;12(12):e1006488. doi: 10.1371/journal.pgen.1006488

Fig 1. ParB-EGFP complexes co-localize with all oriCs in multigenomic S. coelicolor vegetative hyphae.

Fig 1

(A) Scheme of FROS cassette localization in the S. coelicolor chromosome. (B) Images of ParB-EGFP (green) and FROS (red) foci in vegetative hyphae of FROS parB-egfp strain (AK113). The hyphal tips are marked with an asterisk, scale bar—1 μm. (C) Co-localization of FROS and ParB-EGFP foci in AK113 strain along the vegetative hyphae and at the tips of hyphae; the percentage of the foci localizing within the given distance is indicated. (D) Correlation between the distance from ParB-EGFP to the tip of hyphae and distance from FROS signal to tip of FROS parB-egfp (AK113) strain hyphae. The scatterplot with a fitted linear model shows data from 16 hyphae measured at 10 minute time intervals. Data were analyzed using a mixed effects model which can compensate for an individual hypha effect and the standard linear model. Results of both models were similar, comparison of Log-likelihoods of both models showed that random effects of individual hyphae were not significant. Correlation was calculated using the Pearson method.