Fig 2. The tip-proximal chromosome follows the extending vegetative hyphae tip.

(A) Time-lapse snapshots of the FROS strain (DJ-NL102) germinating spore (top panel) and vegetative hypha (bottom panel). The images are the overlay of TetR-mCherry fluorescence (red) and DIC image (gray) (for separate images of TetR-mCherry fluorescence and DIC see S3D Fig). The arrows indicate: red—oriC1 (closest to the tip of the hypha), yellow—oriC2, purple—oriC3, asterisks indicate the tip of outlined hyphae, scale bar—1 μm. (B) Positions of the FROS complexes in the extending hyphae of FROS strain (DJ-NL102). Grey bars are representations of the extending hyphae with 95% confidence interval for hyphal length and semitransparent colored dots represent oriC positions (red–oriC 1, yellow–oriC 2, purple–oriC 3, as shown in the schematic drawing at the right), colored lines indicate 95% mean confidence intervals (analyzed for 41 hyphae). Inset: Distribution (shown as probability density function) of the distances between the hyphal tip and the oriC 1 (red), oriC 2 (yellow) and oriC 3 (purple). (C) Correlation of hyphal extension rate and FROS complex movement calculated for 8 subsequent oriCs from the tip. Scatterplots with fitted linear models show data from 20 hyphae measured at 10 minute time intervals, grey area indicates 95% confidence interval for the model. Minus sign means that the distance between the chromosome and the tip is increasing and a plus sign that it is decreasing. Data were analyzed using a mixed effects model, which can compensate for the effect of individual hyphae and a standard linear model. Results of both models were very similar, comparison of Log-likelihoods of both models showed that random effects of individual hyphae were not significant. Inset: the calculated correlation in relation to the oriC position in the hyphae with a fitted linear model. Correlations were calculated using Pearson method with 95% confidence intervals.