Table 5. RT-PCR validation of selected genes from microarray data.
| Function | Gene | Fold | Microarray |
|---|---|---|---|
| Activation of nuclear factor-kappa β inducing kinase activity | Chi3l1 | -5.5 | -1.5 |
| Carbohydrate metabolic process | Gck | -6.3 | -3.1 |
| Ceramide de novo synthesis | Sptlc3 | -3.0 | -1.7 |
| Cholesterol synthesis | Cyp51 | 5.2 | 5.6 |
| Cholesterol metabolic process | Sqle | 24.4 | 13.5 |
| Fatty acid β-oxidation | Acsl3 | 1.6 | 1.5 |
| Glucose homeostasis | Mlxipl (ChREBP) | -2.6 | -1.6 |
| ChREBPα | -3.2 | -1.6 | |
| ChREBPβ | -2.4 | -1.6 | |
| Immune system | Rorc | -1.9 | -1.5 |
| Cfd | -3.2 | -2.4 | |
| Stress responsive cytokine | Gdf15 | -3.1 | -2.0 |
| Triglyceride metabolic process | Plin4 | -7.6 | -1.5 |