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. 2016 Dec 15;11(12):e0167958. doi: 10.1371/journal.pone.0167958

Fig 8. RT-qPCR validation of transcript abundance from RNA-seq analysis.

Fig 8

(A) Relative expression of eight genes based on actin as the reference housekeeping gene. Y-axis is shown in log base 2 scale for clearer representation of genes with low expression values. (B) Correlation analysis of relative gene expression values from RT-qPCR and RNA-seq analysis. Genes studied include adenosylhomocysteinase (AHCY), chitinase (CHI), ethylene responsive transcription factor (ERF), MYB transcription factor (MYB), selenium binding protein (SBP), thaumatin-like protein (TLP), glutamate dehydrogenase (GDH) and sucrose synthase (SUS). The relative expression for RT-qPCR was calculated by using the 2-ΔCt method based on the reference gene, actin; whereas the relative expression values for RNA-seq are FPKM ratios of individual genes relative to that of actin.