Skip to main content
. 2016 Dec 15;11(12):e0168027. doi: 10.1371/journal.pone.0168027

Fig 5. Combination therapy of Euphorbia kansui and SAHA or JQ1 reactivates latent HIV and activates T cells.

Fig 5

Human primary CD4+ T cells were activated and expanded with CD3/CD28 beads and 30 U/ml IL-2/ml for 7 days. Cells were infected with VSV-G-pseudotyped NL4.3 HSA R+E- for 24 hours. Infectious virus was removed and cells were maintained over 12 days with decreasing concentrations of IL-2 to establish latency. Uninfected cells were maintained in the same conditions, for uninfected controls and to determine T cell reactivation. At 12 days post infection, cells were stimulated for 24 hours with DMSO, PMA (2 μg/ml) and PHA (10 ng/ml), IngDB (50 ng/ml), Euphorbia kansui (50 μg/ml), Euphorbia kansui (50 μg/ml) and SAHA (0.5 μM), or Euphorbia kansui (50 μg/ml) and JQ1 (0.1μM). (a) CD69 expression was measured by flow cytometry. (b) HSA expression was measured by flow cytometry. Data are representative of 3 healthy donors (**p<0.01, ***p<0.001).