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. 2016 Dec 15;11(12):e0168255. doi: 10.1371/journal.pone.0168255

Fig 5. GSK-3β inhibition activates NFAT.

Fig 5

Cardiac myocytes were co-transfected with NFAT-Luc and Renilla luciferase plasmids. (A) Cells were pretreated with 1-Azk for 30 min (1 μM) prior to 100 nM testosterone stimulation for 24 h. (B) Cardiac myocytes were transfected with either siRNA-GSK-3β or a non-targeted siRNA control and then stimulated with 100 nM testosterone for 24 h. (C) Cardiac myocytes were transfected with either GSK-3βWT or GSK-3βS9A, and then stimulated with 100 nM testosterone for 24 h (n = 6 for each condition). Values are presented as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. control; # p < 0.05 vs. 1-Azk.