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. 2016 Dec 15;11(12):e0168320. doi: 10.1371/journal.pone.0168320

Fig 5. Knockdown of LGR4 downregulates the expression of cell cycle-related molecules reminiscent of miR-34a mimic transfection.

Fig 5

(A) After transfection with either LGR4 specific siRNA or miR-34a mimic, ARPE-19 cell lysates were prepared and probed with anti-LGR4, E2F1, p-CDC2, CDK2, CDK4 and CDK6 antibodies. β-actin was probed as a loading control. These results are representative of at least three independent experiments. (B) The band intensity was measured with ImageJ software. The densitometric ratio between proteins of interest and β-actin was calculated and then normalized to the expression level in mock-treated cell. Results were expressed as mean ± SD (n = 3, *p < 0.05).