Table 1.
Primers used in the present study for PCR amplification and sequencing.
| Target gene or scope | Primer set | Sequence (5′ to 3′) | Fragment length | Reference |
|---|---|---|---|---|
| 16S rRNA gene | 27f 1492r |
AGAGTTTGATCHTGGCTCAG | 1465 bp | (12) |
| TACGGYTACCTTGTTACGACTT | ||||
| pmoA | A189f A682r |
GGNGACTGGGACTTCTGG | 525 bp | (18) |
| GAASGCNGAGAAGAASGC | ||||
| A189f mb661r |
GGNGACTGGGACTTCTGG | 510 bp | (9) | |
| CCGGMGCAACGTCYTTACC | ||||
| mmoX | mmoXA mmoXD |
ACCAAGGARCARTTCAAG | 790 bp | (2) |
| CCGATCCAGATDCCRCCCCA | ||||
| mmoX206F mmoX866R |
ATCGCBAARGAATAYGCSCG | 719 bp | (22) | |
| ACCCANGGCTCGACYTTGAA | ||||
| Sequencing | T7 | TAATACGACTCACTATAGGG | variable | Invitrogen |
| Sequencing | T3 | ATTAACCCTCACTAAAGGGA | variable | Invitrogen |