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. 2016 Oct 28;31(4):418–426. doi: 10.1264/jsme2.ME15195

Table 2.

Rhodanobacter thiooxydans proteins involved in lipid metabolism and ketogenesis found after a 5- or 30-d incubation

Uniprot accession number Putative function Abundance (NSpC)a Predicted cellular localizationb

5 d 30 d
I4VIC2 acyl carrier protein 1.44 I
I4VRU7 3-hydroxyacyl-CoA dehydrogenase 0.28 I
I4VV45 YdiF, acetate-CoA transferase 0.13 I
I4VY74 3-oxoacyl-ACP reductase 0.07 I
I4VZY3 acetoacetyl-CoA reductase 0.06 E
I4W091 acyl-CoA dehydrogenase domain-containing protein 0.04 I
I4WAL3 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase 0.08 E
I4WAL4 β-ketoacyl-[ACP] synthase I 0.12 0.43 E
I4WAZ2 acetoacetyl-CoA thiolase 0.66 0.66 E
I4WB09 3-hydroxybutyryl-CoA dehydratase 0.13 I
I4WB55 long-chain fatty acyl CoA ligase 0.07 I
I4WBU8 acetyl-coenzyme A synthetase 0.11 0.12 I
I4WBY7 3-hydroxybutyrate dehydrogenase 0.09 E
I4WBZ6 acetyl-CoA acetyltransferase 0.13 0.07 E
I4WCN8 GpsA, glycerol-3-phosphate dehydrogenase 0.13 0.07 E
I4WDA0 acyl-CoA dehydrogenase 0.04 0.25 I
I4WE25 fatty acid binding protein 0.15 0.09 I
I4WE72 branched-chain alpha-keto acid dehydrogenase subunit E2 0.38 0.22 I
I4WEJ2 FabZ, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase 0.29 I
I4WEK0 acetyl-coenzyme A carboxylase carboxyl transferase 0.04 I
I4WFP4 fatty acyl-CoA synthetase 0.16 0.05 E
I4WGM2 lipase 0.14 0.09 E
I4WIC3 acetyl-CoA acetyltransferase 0.23 0.62 I
I4WIC4 3-hydroxyacyl-CoA dehydrogenase 0.20 0.94 I
I4WJC3 polyhydroxyalkanoate depolymerase 0.07 I
I4WKE0 poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit 0.14 0.05 I
I4WKE1 poly(R)-hydroxyalkanoic acid synthase subunit 0.05 E
I4WKH0 3-oxoacyl-[acyl-carrier-protein] synthase 0.12 0.21 I
I4WKH1 acyl carrier protein 0.11 I
I4WKH2 3-ketoacyl-[acyl-carrier-protein] reductase 0.20 0.04 E
I4WKH3 malonyl-CoA-acyl carrier protein transacylase 0.14 0.31 E
I4WKH4 3-oxoacyl-[acyl-carrier-protein] synthase 0.16 0.13 I
I4WKP8 acyl-CoA dehydrogenase 0.04 I
I4WM78 acyl-CoA dehydrogenase domain-containing protein 0.12 I
I4WM94 acyl-CoA dehydrogenase 0.06 E
I4WM95 acyl-CoA dehydrogenase 0.03 I
I4WM96 acetoacetate decarboxylase 0.20 0.13 E
I4WMY1 phasin 1.86 5.43 I
I4WP46 trans-2-enoyl-CoA reductase 0.11 0.25 I
I4WPL0 enoyl-CoA hydratase 0.19 0.20 I
I4WPU1 acyl-CoA dehydrogenase 0.05 E
I4WR42 acyl carrier protein 0.10 0.35 I
I4WR77 enoyl-CoA hydratase 0.08 I
I4WSC3 lipase 0.08 0.20 E
I4WSG8 acyl-CoA dehydrogenase 0.37 1.02 I
I4WU86 acyl-CoA thiolesterase 0.16 I
I4WUC2 lipase 0.07 E
I4WUD4 acetoacetyl-CoA reductase 0.17 E
I4WZ09 YdiF, acetate-CoA transferase 0.13 I
M4NHA9 acyl-CoA dehydrogenase 0.36 I
a

Data are the mean of two replicates.

b

E: extracellular; I: intracellular.

c

—: protein not detected or protein identification not considered valid.