Figure 3. EJC proteins associate with nascent transcripts of both intron and intronless genes.

(A) Immunolocalization of GFP-LacI (red band in boxed areas) at transgene insertion site: S118 (intron plus) on the X (panel I) and of S136 (intron minus) on the 3L (III) chromosome arm. Bands were mapped at 3B for S118 (II) and 63B for S136 (IV) using a standard polytene chromosome map shown above. (B) DAPI-stained (gray) segments of the X chromosome encompassing 3B (I, II) and 3L chromosome encompassing 63B (III, IV), without (I, III) or with (II, IV) ecdysone treatment, which produces a distinct puff at the transgene insertion locus. (C) Immunolocalization of RNA Pol II Ser2 (red) and eIF4AIII (green) at 3B (I-IV and IX-XII) and 63B (V-VIII and XIII-XVI) loci in S118 (I-VIII) and S136 (IX-XVI) transgene following ecdysone treatment. As there is no insert at locus 63B in S118 (V-VIII) and at 3B in S136 (IX-XII), these are used as ecdysone-unresponsive control loci for the transgene at 63B in S136 (XIII-XVI) and for 3B in S118 (I-IV), respectively. Scale bar represents 20 µm length.

DOI: http://dx.doi.org/10.7554/eLife.19881.010

Figure 3—figure supplement 1. Y14 associates with nascent transcript of both intron and intronless gene reporters.

Immunolocalization of RNA Pol II Ser2 (red) and Y14 (green) at an intro-containing gene reporter (S118 construct at 3B) (I-IV and IX-XII) and intronless counterpart (S136 at 63B) (V-VIII and XIII-XVI). Both genes were induced by ecdysone treatment as described in Material and methods. As there is no insert at locus 63B in S118 (V-VIII) and at 3B in S136 (IX-XII), these are used as ecdysone-unresponsive control loci for the transgene at 63B in S136 (XIII-XVI) and for 3B in S118 (I-IV), respectively.

DOI: http://dx.doi.org/10.7554/eLife.19881.011