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(A) Western blotting of total cell lysate from S2 cells to detect eIF4AIII, Y14, MAGO, following eIF4AIII-RNAi, MAGO-RNAi, Y14-RNAi and in untreated cultures. Tubulin was detected as loading control. (B) Real-time PCR quantification of the mRNA level of indicated transcripts was carried out in MAGO-RNAi (left), Y14-RNAi (middle) and eIF4AIII-RNAi (right) along with untreated S2 cells.
