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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Int J Biol Macromol. 2010 May 25;47(2):104–108. doi: 10.1016/j.ijbiomac.2010.05.014

Fig. 3.

Fig. 3

Cleavage of RPE65 in vitro. 100–1000 µM of H2O2 cleaved RPE65 in vitro. Bovine RPE cells were treated with various amounts of H2O2 in 1 h. Proteins were separated by SDS-PAGE and RPE65 was visualized by western blotting using anti-RPE65 peptide antibody. RPE45 and 20 kDa bands appeared in a dose-dependent manner. 100 µMof H2O2 showed clear 45 and 20 kDa bands, whereas 300, 500 and 1000 µM showed two additional bands around 15 kDa.