Membrane association of RPE65 and RPE45. Proteins from bovine RPE cells was incubated pH= 7.5 PBS buffer (Lanes 1 and 2) or hydroxylamine (Lanes 3 and 4). Samples were centrifuged (175,000 × g) and proteins were separated by SDS-PAGE and proteins were visualized by western blot using RPE65 antibody. Lane 1, pH= 7.5, pellet; Lane 2, pH= 7.5, supernatant; Lane 3, NH2OH, pellet and Lane 4, NH2OH, supernatant.