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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Int J Biol Macromol. 2010 May 25;47(2):104–108. doi: 10.1016/j.ijbiomac.2010.05.014

Fig. 5.

Fig. 5

Membrane association of RPE65 and RPE45. Proteins from bovine RPE cells was incubated pH= 7.5 PBS buffer (Lanes 1 and 2) or hydroxylamine (Lanes 3 and 4). Samples were centrifuged (175,000 × g) and proteins were separated by SDS-PAGE and proteins were visualized by western blot using RPE65 antibody. Lane 1, pH= 7.5, pellet; Lane 2, pH= 7.5, supernatant; Lane 3, NH2OH, pellet and Lane 4, NH2OH, supernatant.