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. 2016 Dec 15;11(12):e0168339. doi: 10.1371/journal.pone.0168339

Fig 5. XBP1 induction following L. infantum infection in U937-derived macrophages.

Fig 5

U937-derived macrophages were infected with L. infantum promastigotes for 6 h or 24 h. As positive control for XBP1 splicing/induction, infected and non-infected cells were treated with 0.5 μg/ml tunicamycin for 6 h. A) XBP1 splicing was not detected using conventional PCR in infected cells. B) A significant induction of both uXBP1 and sXBP1 was shown after 24 h of infection by qPCR, while tunicamycin treatment induced an increase in sXBP1 and a decrease in the uXBP1. ** p < 0.01. TUN, tunicamycin; L, 100 bp DNA ladder; NTC, no template control.