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. 2016 Dec 15;11(12):e0168339. doi: 10.1371/journal.pone.0168339

Fig 7. Xbp1 induction following L. infantum infection in murine macrophages.

Fig 7

Murine macrophages were infected with L. infantum promastigotes for 24 h. As positive control for Xbp1 splicing/induction, infected and non-infected cells were treated with 2 μg/ml tunicamycin for 4 h. A) Xbp1 splicing was not detected using conventional PCR in infected cells. B) A significant induction of sXbp1 was shown after 24 h infection or tunicamycin treatment by qPCR. ** p < 0.01. TUN, tunicamycin; L, 100 bp DNA ladder; NTC, no template control.