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. 2016 Dec 15;11(12):e0168339. doi: 10.1371/journal.pone.0168339

Table 1. Human qPCR primers.

Target mRNA Accession number Forward primer (5’-3’) Reverse primer (5’-3’) Ref
DDIT3 (CHOP) NM_001195053 GGAGCATCAGTCCCCCACTT TGTGGGATTGAGGGTCACATC [22]
HSPA5 NM_005347 CCCCGAGAACACGGTCTTT CAACCACCTTGAACGGCAA [23]
ATF3 NM_001674 GCCATCCAGAACAAGCACCT GGCTACCTCGGCTTTTGTGAT [23]
ATF4 NM_001675 CCCTTCACCTTCTTACAACCTC TGAAGGAGATAGGAAGCCAGAC
MAP1LC3B NM_022818 AAACGGGCTGTGTGAGAAAAC TGAGGACTTTGGGTGTGGTTC [23]
CEBPB NM_005194 CGAAGTTGATGCAATCGGTTT TTAAGCGATTACTCAGGGCCC [23]
CHAC1 NM_024111 TGGTGACGCTCCTTGAAGATC GCACTGCCTCTCGCACATT [23]
sXBP1 NM_001079539 CTGAGTCCGCAGCAGGT TGTCCAGAATGCCCAACAGG
uXBP1 NM_005080 CCGCAGCACTCAGACTACG TGTCCAGAATGCCCAACAGG
B2M NM_004048 ACTGAATTCACCCCCACTGA CCTCCATGATGCTGCTTACA [24]
GAPDH NM_002046 CCATGTTCGTCATGGGTGTG GGTGCTAAGCAGTTGGTGGTG [23]
GUSB NM_000181 GCTACTACTTGAAGATGGTGATCG AGTTAGAGTTGCTCACAAAGGTC
HPRT1 NM_000194 TATGCTGAGGATTTGGAAAGGG AGAGGGCTACAATGTGATGG [25]

DDIT3, DNA-damage-inducible transcript 3; HSPA5, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); ATF3, activating transcription factor 3; ATF4, Activating Transcription Factor 4; MAP1LC3B, microtubule-associated protein 1 light chain 3 beta; CEBPB, CCAAT/enhancer binding protein (C/EBP), beta; CHAC1, ChaC Glutathione-Specific Gamma-Glutamylcyclotransferase 1; sXBP1, X-Box Binding Protein 1, transcript variant 2 (spliced); uXBP1, X-Box Binding Protein 1, transcript variant 1 (unspliced); B2M, Beta-2-Microglobulin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GUSB, Glucuronidase, Beta; HPRT1, Hypoxanthine Phosphoribosyltransferase 1.