Table 1. Human qPCR primers.
Target mRNA | Accession number | Forward primer (5’-3’) | Reverse primer (5’-3’) | Ref |
---|---|---|---|---|
DDIT3 (CHOP) | NM_001195053 | GGAGCATCAGTCCCCCACTT | TGTGGGATTGAGGGTCACATC | [22] |
HSPA5 | NM_005347 | CCCCGAGAACACGGTCTTT | CAACCACCTTGAACGGCAA | [23] |
ATF3 | NM_001674 | GCCATCCAGAACAAGCACCT | GGCTACCTCGGCTTTTGTGAT | [23] |
ATF4 | NM_001675 | CCCTTCACCTTCTTACAACCTC | TGAAGGAGATAGGAAGCCAGAC | |
MAP1LC3B | NM_022818 | AAACGGGCTGTGTGAGAAAAC | TGAGGACTTTGGGTGTGGTTC | [23] |
CEBPB | NM_005194 | CGAAGTTGATGCAATCGGTTT | TTAAGCGATTACTCAGGGCCC | [23] |
CHAC1 | NM_024111 | TGGTGACGCTCCTTGAAGATC | GCACTGCCTCTCGCACATT | [23] |
sXBP1 | NM_001079539 | CTGAGTCCGCAGCAGGT | TGTCCAGAATGCCCAACAGG | |
uXBP1 | NM_005080 | CCGCAGCACTCAGACTACG | TGTCCAGAATGCCCAACAGG | |
B2M | NM_004048 | ACTGAATTCACCCCCACTGA | CCTCCATGATGCTGCTTACA | [24] |
GAPDH | NM_002046 | CCATGTTCGTCATGGGTGTG | GGTGCTAAGCAGTTGGTGGTG | [23] |
GUSB | NM_000181 | GCTACTACTTGAAGATGGTGATCG | AGTTAGAGTTGCTCACAAAGGTC | |
HPRT1 | NM_000194 | TATGCTGAGGATTTGGAAAGGG | AGAGGGCTACAATGTGATGG | [25] |
DDIT3, DNA-damage-inducible transcript 3; HSPA5, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); ATF3, activating transcription factor 3; ATF4, Activating Transcription Factor 4; MAP1LC3B, microtubule-associated protein 1 light chain 3 beta; CEBPB, CCAAT/enhancer binding protein (C/EBP), beta; CHAC1, ChaC Glutathione-Specific Gamma-Glutamylcyclotransferase 1; sXBP1, X-Box Binding Protein 1, transcript variant 2 (spliced); uXBP1, X-Box Binding Protein 1, transcript variant 1 (unspliced); B2M, Beta-2-Microglobulin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GUSB, Glucuronidase, Beta; HPRT1, Hypoxanthine Phosphoribosyltransferase 1.