Table 2. Mouse qPCR primers.
Target mRNA | Accession number | Forward primer (5’-3’) | Reverse primer (5’-3’) | Ref |
---|---|---|---|---|
Ddit3 | NM_007837 | GAGTCCCTGCCTTTCACCTT | TTCCTCTTCGTTTCCTGGGG | |
Hspa5 | NM_001163434 | TCCGGCGTGAGGTAGAAAAG | GGCTTCATGGTAGAGCGGAA | |
Atf3 | NM_007498 | CTCTCACCTCCTGGGTCACT | TCTGGATGGCGAATCTCAGC | |
Atf4 | NM_001287180 | GCAGTGTTGCTGTAACGGAC | ATCTCGGTCATGTTGTGGGG | |
Cebpb | NM_009883 | ACCGGGTTTCGGGACTTGA | TTGCGTCAGTCCCGTGTCCA | |
Chac1 | NM_026929 | TATAGTGACAGCCGTGTGGG | GCTCCCCTCGAACTTGGTAT | |
sXbp1 | NM_001271730 | CTGAGTCCGCAGCAGGT | TGTCCAGAATGCCCAAAAGG | |
uXbp1 | NM_013842 | CCGCAGCACTCAGACTATG | TGTCCAGAATGCCCAAAAGG | |
B2m | NM_009735 | TGCTATCCAGAAAACCCCTCAA | GGATTTCAATGTGAGGCGGG | |
Gapdh | NM_001289726 | TGCCCCCATGTTTGTGATG | TGTGGTCATGAGCCCTTCC | [26] |
Ddit3, Mus musculus DNA-damage inducible transcript 3; Hspa5, Mus musculus heat shock protein 5; Atf3, Mus musculus activating transcription factor 3; Atf4, Mus musculus activating transcription factor 4; Cebpb, Mus musculus CCAAT/enhancer binding protein (C/EBP), beta; Chac1, Mus musculus ChaC, cation transport regulator 1; sXbp1, Mus musculus X-Box Binding Protein 1, transcript variant 2 (spliced); uXbp1, Mus musculus X-Box Binding Protein 1, transcript variant 1 (unspliced); B2m, Mus musculus Beta-2-Microglobulin; Gapdh, Mus musculus glyceraldehyde-3-phosphate dehydrogenase.