Fig 1. The structure of the IZ detection program including: (I) Pre-Processing, (II) Segmentation, (III) Pruning, (IV) Region identification and (V) Innervation points detection.

EMG matrix preparation extracts an appropriate epoch of data for image conversion. Graph-Cut algorithm was used for image segmentation. Parameters (Slope, center/edge coordinates) in step IV were estimated to consider the interaction between regions in the image.