Table 3.
Morphology, Immunophenotype, EBER-ISH and T-cell clonality by PCR in ANKL, EBV-
| Case No. | Biopsy site (s) | Tumor cell morphology | HPS | CD2 | CD3 | CD4 | CD8 | CD5 | CD7 | CD56 | CD30 | βF1 | TCRγ | GZB | LMP1 | EBER | PCR TRG |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Bone marrow | monotonous, medium size | − | na | + c | na | + | − | na | + | na | − | − | + | − | − | No amp |
| 2 | Bone marrow, Liver, PB | monotonous, medium size, azurophilic granules | + | na | + c | − | − | − | na | + | + | na | − | + | − | − | PC |
| 3 | Bone marrow, Omentum, PB | monotonous, small- medium size, azurophilic granules | + | + | + c | − | + | na | + | + | na | na | na | na | na | − | na |
| 4 | Bone marrow | pleomorphic, range in cell size | − | + | − | − | + f,w | − | − | + | − | na | − | + | na | − | na |
| 5 | LN axilla, PB | monotonous, medium size, azurophilic granules | + | + | + c | − | − | − | − | + f | + | − | − | + | − | − | PC |
| 6 | Skin, PB | monotonous, medium size, azurophilic granules | − | + | +c | − | − | − | − | + | +w | − | − | + | − | − | PC |
| 7 | Skin, Liver, Bone marrow, PB | monotonous, medium size, azurophilic granules | − | + | −s | − | − | − | − | + | +f | − | − | + | na | − | PC |
EBER - Epstein–Barr virus-encoded small RNAs; ISH - in situ hybridization
BM - Bone marrow; PB - peripheral blood; LN - lymph node; HPS - hemophagocytic syndrome; Ly -lymphocytes
GZB - granzyme B; PCR - polymerase chain reaction; TRG - T-cell receptor γ chain gene
No amp - no amplification; PC - polyclonal
na - not available; c - cytoplasmic; f - focal; w - weak; s-surface