Table 1.
Mutations present in attenuated DR13 strain. Full length genomes of virulent (GenBank JQ023161) and attenuated DR13 (GenBank JQ023162) viruses were compared with those of reference PEDV strains AH2012 (GenBank KC210145), CV777 (GenBank AF353511) and CO-2013 (GenBank KF272920).
| Point Mutations |
Deletions |
||||
|---|---|---|---|---|---|
| Gene | Totala | Non-silent | Uniqueb | nt | Resultc |
| 5′ UTR | 6 | – | – | ||
| Nsp1 | 4 | 1 | – | ||
| Nsp2 | 64 | 30 | 6 | ||
| Nsp3 | 14 | 30 | 12 | 23 | Δ1036–1043 |
| Nsp4 | 27 | 6 | 5 | ||
| Nsp5 | 24 | 6 | 2 | ||
| Nsp6 | 22 | 2 | 1 | ||
| Nsp7 | 4 | – | – | ||
| Nsp8 | 6 | 3 | – | ||
| Nsp9 | 9 | – | – | ||
| Nsp10 | 2 | – | – | ||
| Nsp12 | 39 | 2 | 2 | ||
| Nsp13 | 39 | 3 | 1 | ||
| Nsp14 | 39 | 2 | 2 | ||
| Nsp15 | 24 | 1 | – | ||
| Nsp16 | 19 | 3 | 1 | ||
| S | 67 | 25 | 25 | 3 | Δ152 |
| ORF3 | 7 | 1 | – | 48 | Δ92–224 |
| E | 5 | 2 | 2 | 21 | Δ23–29 |
| M | 13 | 4 | 2 | ||
| N | 40 | 13 | 3 | ||
| 3′UTR | 2 | – | – | ||
a
All mutations in attenuated DR13 compared with parental DR13 are included.
b
Non-silent mutations present exclusively in attenuated DR13 genome.
c
Aminoacid position in the replicase polyprotein is indicated for Nsp3.