Tail suspension test to assess depression-like behavior in Dio3 −/− mice. Dio3 +/+ and Dio3 −/− mice (Dartmouth cohort) were tested in the tail suspension test. The latency to immobility was measured (A). The number of episodes (B), the total time (C) and the average time per episode (D), when the mice remained still were analyzed. Data represent the mean ± SEM of results from 6 to 9 mice (per genotype and sex) representing at least three different litters. *, **, ***, indicate P<0.05, P<0.01, P<0.001, respectively, Dio3 +/+ vs Dio3 −/−, as determined by two-way ANOVA followed by Sidak’s test.