Table 1.

Dilutions, product numbers, and protocols used for the antibodies utilized in this study

Gene	Company	Product #	Fixation	Blocking	Secondary	Dilution
DRAQ5	Biostatus Limited	DR50200	acetone rmethanol or 4% PFA in 1xPBS	2% BSA or 5% goat serum		1:2000
αSMA	SigmaAldrich	F3777	acetone rmethanol or 4% PFA in 1xPBS	2% BSA or 5% goat serum	FITC conjugated	1:250
E-cadherin	Cell Signaling	4065	4% PFA in 1xPBS	5% goat serum	anti-rabbit	1:100
Aquaporin 0	Millipore	ab3071	acetone rmethanol	2% BSA	anti-rabbit	1:200
Pax6	Millipore	AB2237	4% PFA in 1xPBS	5% goat serum	anti-rabbit	1:200
c-Maf	Santa Cruz Biotechnology	sc7866	acetone rmethanol	2% BSA	anti-rabbit	1:100
Prox1	Belecky-Adams et al., 1997; Duncan et al., 2002		acetone rmethanol	2% BSA	anti-rabbit	1:500
pErk	Cell signaling	scl0572	acetone rmethanol	2% BSA	anti-goat	1:200
pAkt	Cell signaling	sc48789	4% PFA in 1xPBS	5% goat serum	anti-rabbit	1:100
Collagen IV	Abeam	AB6586	acetone rmethanol	2% BSA	anti-rabbit	1:200
β1-integrin	Millipore	MAB1997	acetone rmethanol	2% BSA	anti-rat	1:200
Laminin	Abcam	11575250	acetone rmethanol	2% BSA	anti-rabbit	1:200
p27kip1	Santa Cruz Biotechnology	sc528	paraffin sections	10% BSA	anti-rabbit	1:50
p57kip2	Santa Cruz Biotechnology	scl039	paraffin sections	10% BSA	anti-rabbit	1:50
β-crystallin	Gift from Dr. Samuel Zigler		paraffin sections	5% goat serum	anti-rabbit	1:100
γ-crystallin	Santa Cruz Biotechnology	sc22415	paraffin sections/ 4% PFA fixation	5% goat serum	anti-goat	1:100
Jagged 1	Santa Cruz Biotechnology	sc8303	4% PFA fixation	5% goat serum	anti-rabbit	1:100
Foxe3	Santa Cruz Biotechnology	scl34536	4% PFA fixation	5% goat serum	anti-rabbit	1:100
Hes1	Santa Cruz Biotechnology	sc25392	4% PFA fixation	2% BSA and 5% goat serum	anti-rabbit	1:100
pSmad 1/5/8	Santa Cruz Biotechnology	scl2353- R	4% PFA fixation	1% BSA and 3% goat serum	anti-rabbit	1:100
pFRS2α-Y436	R&D Systems	AF5126	4% PFA fixation	2% BSA and 5% goat serum	anti-rabbit	1:100