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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Hypertension. 2016 Oct 31;68(6):1494–1503. doi: 10.1161/HYPERTENSIONAHA.116.08171

Figure 6. An alpha globin mimetic peptide significantly reduces hyperconstriction in arterioles from human patients with hypertension.

Figure 6

(A) Far-Western blotting for recombinant human eNOS oxygenase domain on a non-denaturing gel using anti-eNOS and biotin-HbαX probes and corresponding secondary antibodies for fluorescent detection. Left lane serves as a molecular weight marker, but it is not used to estimate molecular weight due to the non-denaturing condition of the gel. The samples were run in duplicate. (B) Western blot of the expression of alpha globin without its beta globin partner is confirmed in human arterioles, and a 10-fold dilution of RBC samples is used as a positive control for alpha and beta globin chains. Note the lack of alpha globin in aorta. Arrowheads for CD31 indicate 100 kDa, and alpha and beta globin indicate 10 kDa. (C) Resistance arterioles (approximately 100 μm in diameter) isolated from human adipose tissue biopsies were cannulated and subject to phenylephrine cumulative dose-response curves in the absence (black) or presence (blue) of 5 μmol/L HbαX. (n=4). * indicates p<0.05, ** indicates p<0.01 using two-way ANOVA and a Bonferroni post-test.