Table 1.
Timeline of procedures for subjects.
Infant Age | Procedure |
---|---|
Day 5 | Removed from group with dam |
Day 6 | First ISO or sham exposure |
Day 9 | Second ISO or sham exposure |
Day 12 | Third ISO or sham exposure |
Day 14 | IBAS test Release back to social group |
Day 30 | IBAS test (removed from social group with dam day before) Release back to social group |
3 months | Free play/Human Intruder/Novel object tests (removed from social group with dam day before) Released back to social group |
12 months | Weaned from natal group; placed in new social group Home cage observations (started approximately 1–2 days after moved to new group) Human Intruder /Novel object test (approximately 1–2 weeks after end of focal observations) |
Abbreviations:
IBAS: Infant Behavior Assessment Scale
ISO: Isoflurane