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. Author manuscript; available in PMC: 2017 Aug 18.
Published in final edited form as: Cell Chem Biol. 2016 Aug 11;23(8):978–991. doi: 10.1016/j.chembiol.2016.06.016

Figure 2. Inz-1 is a fungal-selective inhibitor of mitochondrial cytochrome bc1.

Figure 2

A) Mutations found in cytochrome B (COB) in two Inz-1-resistant isolates, visualized by the Integrative Genomics Viewer (IGV). B) Inhibition of yeast cytochrome B enzymatic activity by Inz-1 in both wild-type and F90Y mutant mitochondria. C) Resistance of F90Y mutant to growth inhibition by Inz-1. D) Fluconazole tolerance of C. albicans CaCi-2 WT, treated with Inz-1, or rip1Δ/Δ deletion mutant. Growth at the indicated concentrations of fluconazole was measured by broth microdilution using OD600 as endpoint. E) Comparison of inhibition of cytochrome B activity in C. albicans and human (HEK293) mitochondria. F) Comparison of the effect of Inz-1 on proliferation of HepG2 and NIH-3T3 cells under respiring conditions (DMEM-galactose) with effect on proliferation of C. albicans in presence of 8 μg/mL fluconazole. Graphs in B-F each represent data pooled from two independent experiments, with error bars depicting S.E.M.