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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Chem Phys Lipids. 2016 Jun 16;200:32–41. doi: 10.1016/j.chemphyslip.2016.06.004

Figure 4. Fluorescence Recovery After Photo-bleaching (FRAP) with TopFluor lipids.

Figure 4

A) The diffusion coefficient was determined from three independent experiments for each TopFluor lipid shown in the plasma membrane of A549 cells. B) The mobile fraction of the four different TopFluor lipids was calculated from photo bleaching of a 1.1 μm radius circle at the plasma membrane followed by analysis in ImageJ and Kaleidagraph. C) Mobile fraction of TopFluor-C1P after FRAP at the plasma membrane or Golgi of A549 cells. D) A normalized fluorescence versus time plot is shown for the FRAP of TopFluor-C1P in the plasma membrane of A549 cells.