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. 2016 Dec 16;10:281. doi: 10.3389/fncel.2016.00281

Figure 4.

Figure 4

Changes in phosphorylated extracellular signal-regulated kinases 1/2 (p-ERK1/2) immunoreactivity in hippocampal regions of mice treated with EP-80317 and exposed to different sessions of 6-Hz corneal stimulation. In (A), p-ERK1/2 immunoreactivity is illustrated in CA1, CA3, and DG, in a representative unstimulated control (ctrl) mouse. Moreover, p-ERK1/2 immunoreactivity is also shown in mice exposed to one or three different sessions of 6-Hz corneal stimulation and pretreated with saline (sal) or EP-80317 (ep). Immunoreactivity was measured and results are illustrated in (B–D). Note that p-ERK1/2 expression was initially increased in CA1 (°P < 0.05 vs. controls; Fisher’s least significant difference test), both in EP-80317 and saline-treated mice. After the third seizure, immunopositive cells increased also in CA3 (C) and DG (D) (°°P < 0.01, °°°P < 0.001 vs. controls) and were higher than in the initial session (#P < 0.05, ##P < 0.01, ###P < 0.001 vs. session 1 of the respectively considered group of treatment). However, the changes observed in mice treated with EP-80317 were less pronounced than in saline-treated mice (***P < 0.001 vs. sal-3). Scale bar, 50 μm.