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Double immunofluorescence confocal laser scanning micrographs of mice exposed to repeated 6-Hz corneal stimulation. Photomicrographs illustrating co-labeling with phosphorylated extracellular signal-regulated kinases 1/2 (p-ERK1/2) (green) and FosB/ΔFosB (red) in CA1 of representative mouse for each group of treatment. Double-label immunofluorescence revealed the coexpression of p-ERK1/2 and FosB/ΔFosB in the experimental groups. Scale bar, 75 μm.
