Fig. 3.

Generation of the D492M cell line. The epithelial cell line D492 was established from normal breast tissue [23]. When cultured in 3D, it forms branching colonies reminiscent of the TDLU. In co-culture with breast endothelial cells, this cell line can form mesenchymal colonies. When branching colonies are isolated from 3D culture and re-plated, they form both branching and mesenchymal colonies during secondary co-culture, while the mesenchymal colonies only give rise to additional mesenchymal colonies. A mesenchymal cell line (D492M) was established from a mesenchymal colony using single cell cloning. Adapted from Sigurdsson et al. [46] with permission