FIGURE 1.

Reducing Hap1 expression decreases the release of insulin. A, insulin levels in blood plasma of KO and WT pups by RIA (upper panel) (**, p < 0.001; KO n = 25,WT n = 30). INS-1 cells treated with Hap1-siRNA displayed an obvious reduction in insulin release compared with control cells (bottom panel) (**, p < 0.001; n = 10). B, immunofluorescent staining of Hap1 in INS-1 cells transfected with Hap1-siRNA or scramble-siRNA. C, Western blotting of INS-1 cells showing that Hap1 protein level was reduced by Hap1-siRNA treatment but not by scramble-siRNA. Scale bars, 20 μm.