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Synthetic oligonucleotides used in site-directed mutagenesis of PHO84WT
| Amino acid substitution | Mutagenized oligonucleotide (5′ → 3′) |
|---|---|
| Tyr179-Ala | ggtattggtatcggtggtgacgccccactatcttctattattac |
| Tyr179-Gly | ggtattggtatcggtggtgacggcccactatcttctattattac |
| Tyr179-Ser | gtattggtatcggtggtgactccccactatcttcta |
| Tyr179-Phe | tagaagatagtgggaagtcaccaccgataccaatac |
