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. 2016 Nov 7;291(51):26478–26486. doi: 10.1074/jbc.M116.745851

FIGURE 1.

FIGURE 1.

Effects of treatment with GAG-degrading enzymes and the addition of GAGs on Bac-PrPres amplification. A, PK- and heat-treated cell lysate (PHCL), chondroitin sulfate B (CSB), and heparin (HP) were digested with chondroitinase ABC (Ch) or heparinase II (Hp). CSB and HP were digested in 1× PBS containing 4 mm EDTA and 0.25% Triton X-100. NT, non-treated sample. Samples were separated using SDS-PAGE, and the gel was stained with Alcian blue to visualize GAGs. B, ME7- and mBSE-seeded PMCA reactions were performed using PHCL digested with chondroitinase ABC, heparinase II, or benzonase (Bz). C, ME7- and mBSE-seeded iPMCA reactions in the presence of nucleic acids were performed by adding polyA (PA), hyaluronic acid (HA), chondroitin sulfate A (CSA), CSB, chondroitin sulfate C (CSC), keratan sulfate (KS), HP, or HS at 50 μg/ml final concentration. NA, no additive.