Skip to main content
. 2016 Nov 7;291(51):26478–26486. doi: 10.1074/jbc.M116.745851

FIGURE 2.

FIGURE 2.

Recovery effects of GAGs on Bac-PrPres amplification inhibited through nucleic acid depletion. ME7-, mBSE-, and Chandler-seeded iPMCA reactions were performed under conditions of nucleic acid depletion by adding PA, HA, CSA, CSB, CSC, keratan sulfate (KS), HP, or HS. PA, HP, and GAGs except for HP were added at 40, 10, and 100 μg/ml, respectively. Moreover, HS and CSB were added to a final concentration of 500 μg/ml for ME7- and Chandler-seeded PMCA (500HS and 500CSB). Ten rounds of serial amplification were conducted. The results of the first, second, third, fifth, and tenth rounds of PMCA are shown. ND, not done (amplification not performed). NA, no additive.