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. 2016 Nov 7;291(51):26529–26539. doi: 10.1074/jbc.M116.753335

TABLE 2.

LC/MS/MS identification of major peptides in α4β3δ GABAAR SDS-polyacrylamide gel bands

Shown are search results from a Homo sapiens protein database: HSPA1A, heat shock 70-kDa protein 1A/1B; PRMT5, arginine N-methyltransferase 5; GABRA4, GABAAR α4 subunit; LMNB1, lamin-B1; GABRD, GABAAR δ subunit; IGF2BP1, insulin-like growth factor 2 mRNA-binding protein 1; ABCD3, ATP-binding cassette subfamily D member 3; GABRB3, GABAAR β3 subunit; TUBA1A, tubulin α-1A chain; TUBB2A, tubulin β-2A chain; DDX47, probable ATP-dependent RNA helicase DDX47.

Gel band Protein (gene name) Peptides detected MS/MS scans Average intensity Coverage
%
78 kDa HSPA1A 68 911 1,450,000 70
PRMT5 40 166 597,000 48
GABRA4 27 127 393,000 46
LMNB1 29 65 253,000 48
72 kDa GABRA4 57 1,005 756,000 55
GABRD 15 60 209,000 29
IGF2BP1 17 42 173,000 31
ABCD3 17 36 118,000 23
62 kDa GABRD 29 487 924,000 38
GABRB3 33 282 723,000 41
GABRA4 32 127 500,000 45
TUBA1A 24 118 336,000 46
58 kDa GABRB3 35 304 1,970,000 40
GABRD 27 421 1,400,000 39
TUBA1A 34 272 956,000 61
GABRA4 28 115 712,000 42
TUBB2A 47 476 655,000 59
54 kDa GABRA4 26 140 1,180,000 41
GABRD 25 393 892,000 40
GABRB3 15 52 639,000 30
DDX47 13 31 176,000 35