Table 1. Kinetic nature of molecular interactions.

Average lifetime of NtrC hexamer	∼4 min
Average time of NtrC's DBD in association with an enhancer	∼12 min
Average time of NtrC's DBD in association with a low-affinity site	∼72 s
Searching time for hexamers at enhancer I to encounter a holoenzyme	3 s, 64 s, 128 s, 640 s and + – separately corresponding to the cases where the II-V bridging is present, none of, one of, two of and all of sites III-V is/are bound by NtrC
Searching time for hexamers at enhancer II to encounter a holoenzyme	80 s, 160 s, 640 s, + – separately corresponding to the cases where none, one, two and all of sites III-V is/are bound by NtrC
Searching time for hexamers at enhancer II to encounter site V	∼16 s, 160 s, + – separately corresponding to the cases where neither, either and both of sites III and IV is/are bound by NtrC
Average lifetime of II-V bridging	∼55 s
Half the maximal occupation of an enhancer	∼2 nM of NtrC dimers
Half the maximal occupation of a low-affinity site	∼400 nM of NtrC dimers