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. 2016 Aug 17;44(22):10960–10973. doi: 10.1093/nar/gkw711

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — vU1 and U1 genes follow reciprocal patterns of expression during differentiation and cell re-programming. (A) qRT-PCR analysis of steady state U1, vU1.8 and vU1.20 levels in human ESCs, EBs, ESC-derived monocytes, human skin fibroblasts and fibroblasts-derived iPSCs. Primers used are illustrated in the schematic above the graph. U1/vU1 levels were estimated using a genomic (g)DNA as standard and normalized to 7SK levels across the different cell types. U1 levels are indicated on the Y-axis to the left of the graph and vU1 levels on the right Y-axis. Error bars represent SEM of three independent differentiation/re-programming experiments (Two-way ANOVA analysis (U1); **** = P < 0.0001 and one-way ANOVA (vU1.8 +vU1.20); ** = P < 0.01, *P = 0.05. (B) The ratio of vU1 to U1 levels, expressed as a percentage of U1 levels, across the different cell types.