Figure 4.

Clr6CII HDAC activity is required to suppress tgp1⁺ expression. (A) The levels of acetyl-histones (H3K9ac) at the tgp1⁺ promoter primer pair 3 (see Figure 1A) were measure by ChIP-qPCR in wild-type cells grown in the presence or absence of phosphate and set2Δ cells grown in the presence of phosphate. (B) RT-qPCR analysis of tgp1⁺ mRNA levels in phosphate-replete wild-type cells and cells lacking either Pst2 or Alp13, each of which are critical HDAC subunits of Clr6CII^Rpd3S. Error bars represent standard deviation resulting from three independent replicates. (C) Northern analysis of tgp1⁺ mRNA levels in wild-type cells grown in the presence or absence of phosphate and in phosphate-replete cells with compromised Clr6CII^Rpd3S activity (pst2Δ).