Figure 6.

Elongation factors involved in co-transcriptional chromatin reorganization are required to suppress pho1⁺ expression. (A) Schematic depiction of the pho1⁺ locus, including the lncRNA prt gene that occupies the pho1⁺ promoter and overlaps the gene body downstream. (B and C) RT-qPCR analysis of pho1⁺ mRNA levels and upstream prt lncRNA levels in wild-type cells grown in the presence or absence of phosphate using primer pairs 1 and 4, respectively (see Figure 7A). Error bars represent standard deviation resulting from at least three independent replicates. (D and E) Northern analysis of tgp1⁺ mRNA levels in wild-type cells grown in the presence or absence of phosphate and in phosphate-replete set2Δ, spt6-1, and spt16-18 cells.