Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 8;44(22):10619–10630. doi: 10.1093/nar/gkw801

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 7. — lncRNA transcription regulates pho1⁺ by transcriptional interference. (A) Schematic depiction of the pho1⁺ locus, including the lncRNA prt gene that occupies the pho1⁺ promoter and overlaps the gene body downstream. Primer pairs spaced over the pho1⁺ locus are displayed below. (B) ChIP-qPCR for Ser2P, the elongating form of RNAPII, over the pho1+ locus in response to changes in phosphate availability. (C) Nucleosome density over the pho1+ locus was measured by histone H3 ChIP-qPCR experiments in wild-type cells grown in the presence or absence of phosphate. (D) ChIP-qPCR for H3K36me3 levels over the pho1+ locus in phosphate-replete and phosphate-starved wild-type cells. (E) Histone density was measured by histone H3 ChIP-qPCR experiments in wild-type cells, Stp6 mutant cells (spt6-1), and FACT mutant cells (spt16-18) grown in the presence of phosphate and at the restrictive temperature of 36°C. Error bars represent standard deviation resulting from at least three independent replicates.