Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 30;44(22):10691–10710. doi: 10.1093/nar/gkw863

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 1. — Schematic illustrations of the non-cooperative and cooperative binding of gp32 monomers to: (A) the 3′-tail-pdT7-Cy3/Cy5 p/t DNA construct; (B) the 3′-tail-pdT15-Cy3/Cy5 p/t DNA construct; and (C) the 3′-tail-pdT22-Cy3/Cy5 p/t DNA construct. The single-stranded regions of these DNA constructs can bind (A) 0 or 1, (B) 0, 1 or 2 or (C) 0, 1, 2 or 3 gp32 proteins, respectively. (D) Bulk fluorescence measurements of the 3′-tail-pdT15-Cy3/Cy5 construct at 100 nM oligomer concentration exhibited changes in the FRET efficiency upon titration with gp32. Red, blue and green curves are partially overlapping. The inset shows the values of calculated from the peak Cy3/Cy5 fluorescence intensities. (E) The results of bulk fluorescence gp32 titration measurements (at 100 mM NaCl, 6 mM MgCl₂ and 10 mM Tris (pH 8.0) concentrations) for initial 100 nM concentrations of six of the p/t DNA constructs used in our studies (for both 5′ and 3′ oliogo-dT tails of length N = 7, 15 and 22).

Inline graphic — Schematic illustrations of the non-cooperative and cooperative binding of gp32 monomers to: (A) the 3′-tail-pdT7-Cy3/Cy5 p/t DNA construct; (B) the 3′-tail-pdT15-Cy3/Cy5 p/t DNA construct; and (C) the 3′-tail-pdT22-Cy3/Cy5 p/t DNA construct. The single-stranded regions of these DNA constructs can bind (A) 0 or 1, (B) 0, 1 or 2 or (C) 0, 1, 2 or 3 gp32 proteins, respectively. (D) Bulk fluorescence measurements of the 3′-tail-pdT15-Cy3/Cy5 construct at 100 nM oligomer concentration exhibited changes in the FRET efficiency upon titration with gp32. Red, blue and green curves are partially overlapping. The inset shows the values of calculated from the peak Cy3/Cy5 fluorescence intensities. (E) The results of bulk fluorescence gp32 titration measurements (at 100 mM NaCl, 6 mM MgCl₂ and 10 mM Tris (pH 8.0) concentrations) for initial 100 nM concentrations of six of the p/t DNA constructs used in our studies (for both 5′ and 3′ oliogo-dT tails of length N = 7, 15 and 22).