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. 2016 Sep 30;44(22):10691–10710. doi: 10.1093/nar/gkw863

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 9. — Representative smFRET trajectories of the 5′-tail-pdT15-Cy3/Cy5 p/t DNA substrate at various gp32 concentrations. (A) p/t DNA substrates alone; (B) in the presence of 0.1 μM gp32; (C) 1.0-μM gp32; and (D) 10 μM gp32. Histograms of the smFRET efficiencies [ = I_A/(I_D + I_A)], which are based on the compilation of thousands of individual smFRET trajectories, are shown in the right column. The trajectory for the DNA substrate alone [shown in Panel (A)] exhibits slow fluctuations between a high-FRET state centered at 0.6 and a low-FRET state centered at 0.35. The red-shaded regions in the histograms show the probability distribution of background signals, which was determined from control measurements performed using biotin-Cy3 samples (see Supplementary Data). The buffer solution in this figure and the next contained 100 mM NaCl, 6 mM MgCl₂ and 10 mM Tris (pH 8.0).

Inline graphic — Representative smFRET trajectories of the 5′-tail-pdT15-Cy3/Cy5 p/t DNA substrate at various gp32 concentrations. (A) p/t DNA substrates alone; (B) in the presence of 0.1 μM gp32; (C) 1.0-μM gp32; and (D) 10 μM gp32. Histograms of the smFRET efficiencies [ = I_A/(I_D + I_A)], which are based on the compilation of thousands of individual smFRET trajectories, are shown in the right column. The trajectory for the DNA substrate alone [shown in Panel (A)] exhibits slow fluctuations between a high-FRET state centered at 0.6 and a low-FRET state centered at 0.35. The red-shaded regions in the histograms show the probability distribution of background signals, which was determined from control measurements performed using biotin-Cy3 samples (see Supplementary Data). The buffer solution in this figure and the next contained 100 mM NaCl, 6 mM MgCl₂ and 10 mM Tris (pH 8.0).