Skip to main content
. 2016 Oct 3;44(22):10744–10757. doi: 10.1093/nar/gkw874

Figure 4.

Figure 4.

Gene conversion between inverted repeats in the centromere occurs in a Rad51- and Rad54-dependent manner. (A) ade6B and ade6X heteroalleles introduced into cen1 are illustrated. The central region (cnt1) is surrounded by a set of inverted repeats (imr1, dg, dh and irc1). Hp, HpaI. The introduction of ade6B/X heteroalleles did not change the sensitivity to a microtubule destabilizing drug, TBZ (Supplementary Figure S6B), suggesting that it does not interfere with centromere function. (B) Spontaneous rate of gene conversion between inverted repeats in the centromere region. Wild-type, rad51Δ, rad54Δ, rad54KA and rad54KR strains (TNF3144, 3257, 3286, 4299 and 4311, respectively) were grown in the presence of adenine, and subsequently Ade+ recombinants were identified on EMM plates. Cells were grown at 33°C.