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. 2016 Oct 3;44(22):10744–10757. doi: 10.1093/nar/gkw874

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Rad51 and Rad54 suppress crossing over between centromere repeats. (A) Crossing over between inverted repeats in cen1. ade6B/X heteroalleles are omitted in the illustration for simplicity. A, AfeI. (B) Chromosomal DNA was prepared from parental and Ade⁺ recombinants, treated with AfeI, and applied to 0.6% agarose gel PFGE (switching time 1 to 6 s, 6 V/cm for 14 h, 10°C) in 0.5x TBE buffer. Asterisk indicates the band derived from cen3. (C) Pie charts show the proportion of COs among Ade⁺ recombinants examined. P-values were determined by the two-tailed Fisher's exact test.