Table 2. G-quadruplex variants with peroxidase activity >20% of the reference construct.

Tetrad sequence	Relative percent peroxidase activity
GGGG	100 ± 0
TGGG	94 ± 9
ATGG	88 ± 6
TCGG	84 ± 16
CCGG	82 ± 13
CTGG	80 ± 4
TTGG	79 ± 8
CGGG	77 ± 6
AAGG	77 ± 8
ACGG	76 ± 7
AGGG	75 ± 8
CAGG	73 ± 8
TAGG	68 ± 4
GTGG	65 ± 6
GGCC	62 ± 7
GCGG	52 ± 1
GGTC	50 ± 8
GAGG	45 ± 25
GAAG	40 ± 4
GCAG	37 ± 5
GGCG	35 ± 8
GGTG	31 ± 8
GGGA	27 ± 4
GATG	27 ± 6
GGAG	27 ± 3
GGGT	26 ± 7
GGCT	26 ± 5
GTAG	25 ± 3
GGGC	25 ± 7
GGTT	23 ± 4

Reported values represent the average ± standard deviation of three experiments. Experiments were performed at 10 μM DNA concentration in a buffer containing 200 mM KCl, 1 mM MgCl₂, 20 mM HEPES pH 8, 0.05% Triton X-100, 0.5 μM hemin, 1% DMSO, 5 mM ABTS, and 600 μM H₂O₂.