FIGURE 2.

SSA causes pre-mRNA leakage in a gene-specific manner. (A–D) HeLa S3 cells were treated with SSA (100 ng mL⁻¹, 6 h) and the cells were disrupted and separated into cytoplasmic and nuclear fractions. RNA samples were purified from the fractions. RT-PCR analysis was performed using the RNA samples to detect spliced and unspliced forms of the genes, of which pre-mRNAs were not affected by SSA (A), accumulated only in the cytoplasm (B), accumulated in both fractions (C), and accumulated only in the nucleus (D). Open triangles indicate spliced mRNA, while the filled triangles indicate pre-mRNA. Asterisk indicates nonspecific bands.