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. 2017 Jan;23(1):86–97. doi: 10.1261/rna.058875.116

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© 2016 Shoji et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Artificial piRNA production from the silkworm Importin-5 gene. (A,B) Artificial piRNAs derived from the Importin-5 gene. The start sites of piRNAs mapped to the Importin-5 gene are indicated. (C,D) Identification of the cleavage site of Importin-5 mRNA by the piRNA-I–BmAgo3 complex. Importin-5 mRNA-derived fragments were amplified by a modified RACE method using total RNA from BmN-4 cells transfected with plasmids expressing pre-piRNA-I (C) or pre-piRNA-I-nc (D). The RACE adaptor and cloned 5′-end sequences are indicated. Twelve 5′-ends were determined, with 11 clones showing identical sequences. (E) Expression level of Importin-5. Expression of Importin-5 mRNA was examined by RT-qPCR. Data shown are mean ± standard deviation (n = 5). Data were subjected to Student's t-test (one-sided). NS, not significant (P = 0.3412).