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. 2017 Jan;23(1):86–97. doi: 10.1261/rna.058875.116

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© 2016 Shoji et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 7. — 3′ Sequences required for target recognition by the piRNA–PIWI complex. (A) Nucleotide sequences of piRNA-I derivatives possessing identical (green) and different (blue) nucleotides to those of a complementary sequence of Importin-5 (red). (B) Expression levels of piRNA-J in BmN-4 cells transfected with pre-piRNA-I derivatives. The piRNA-J amounts were measured by RT-qPCR and normalized to those of let-7. Data shown are mean ± standard deviation (n = 4). (*) P < 0.05 with the Mann-Whitney U test followed by the Benjamini-Hochberg correction method to control the false discovery rate.