Figure 6.

Characterization of antibodies raised against the carboxy terminal of Aplysia Classical Calpain (A) and Aplysia SOL calpain (B). Classical calpain and SOL calpain were purified from SF9 cells that were transduced with baculovirus encoding the His-tagged Classical calpain or the His-tagged SOL calpain. Nervous system homogenate was prepared as described in Materials and Methods and 10 µg of total protein were loaded per lane. Predicted molecular weights based on the cloned calpains are indicated. (C,D) Overexpression of (C) the dominant-negative Classical calpain (cells from Fig. 5B) and (D) the dominant-negative SOL calpain (cells from Fig. 5C) was confirmed using immunocytochemistry with the antibodies raised against the Classical calpain and the SOL calpain, respectively.